Coding

Part:BBa_K1946005:Design

Designed by: Ayşe Naz Ozantürk   Group: iGEM16_Bilkent-UNAMBG   (2016-10-13)


XylR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1665
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1082
    Illegal BsaI.rc site found at 1460


Design Notes

The sequence was codon optimized for E.coli K12.


Source

The source translation sequence can be accessed at GenBank: AAB59162.1

References

Huang WE, Singer AC, Spiers AJ, Preston GM, Whiteley AS. (2008) Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environ Microbiol. 2008;10(7):1668-80.

Zhang Y-T, Jiang F, Tian Z-X, Huo Y-X, Sun Y-C, et al. (2014) CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive s54-Promoter Pu in Escherichia coli. PLoS ONE 9(1): e86727. doi:10.1371/journal.pone.0086727